Films made of bio-inspired microstructured arrays hold great promise for many applications that require customized properties, such as energy, aerospace, marine, biomedical, optics, and soft robotics. However, reliable, fast and scalable production of such films remains a great challenge. This research reports a novel, fast, and cost-effective approach to building scalable films characterized by multiscale bio-inspired microstructures using single-layer photopolymerization (SLP). The proposed SLP process utilizes digital light patterns that provide a customizable approach toward a wide range of high-quality and diverse morphologies, which could facilitate pre-programmed properties. Our technique harnesses photopolymerization principles with a unified digital masking system to manipulate the target microstructure profile and generate multiscale arrays within seconds that transcend traditional layer-by-layer manufacturing constraints. The combined effect of spatially varied light patterns and energy dose enables remarkable control over microstructure topology and distribution without requiring any pre-/post processing. This paper first introduces the novel SLP process, setup, and the mechanisms of forming microstructures. Then, the effect of process parameters on the microstructure profile was characterized and modeled. The physical and mechanical properties of fabricated arrays were compared with traditional layer-by-layer techniques using scanning electron microscopy and compressive tests. The proposed SLP process not only enhanced the surface quality but also increased the mechanical performance, owing to layer-less solidification. To validate the efficiency and feasibility of the proposed approach, films consisting of various bio-inspired multidimensional microstructures were fabricated, including moth eye-inspired antireflective structures (zero-dimension), mosquito-inspired microneedle array (one-dimension) and shark skin-inspired drag-resistant coatings (two-dimension). The results indicate that the single-step SLP approach can facilitates the creation of flexible arrays that maintain structural integrity across various scales, making it suitable for both microscale and macroscale fabrication, paving partway for advancing research in materials science, surface engineering, and industrial applications.