This study explores the fabrication of barbed sutures of biodegradable polymers, such as P4HB and Cagut, using femtosecond laser. Barbed sutures are on high demand for minimally invasive procedures, with the benefits of reducing the need for knots, enhancing would closure stability and minimizing tissue trauma. Traditional approaches, such as mechanical cutting and longer-pulses lasers, results in imprecise cutting and extended thermal damage, whereas ultrashort pulse durations of femtosecond lasers enable high precision cutting, with the added benefits of minimal heat-affected zones. This research investigates the effects of key laser parameters, such as laser fluence, repetition rate, overlapping ration and scanning passes, on barb quality and identifies the optimal conditions for consistent, high-quality barbs with sharp tips and minimal thermal damage. Moreover, the threshold fluence values established here, for P4HB and Catgut, serve as a reference for future study. Results demonstrate that femtosecond laser technology can be a promising alternative to traditional barb fabrication techniques.